HEPATOPROTECTIVE ACTIVITY OF Abelmoschus manihot L. Medik LEAVES EXTRACT AND ITS NANOPARTICLE AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN RATS
Abelmoschus manihot L. Medik leaves used as food and traditional medicine. Leaves decoction water is used to treat various diseases. Flavonoids content in the leaf such as hyperoside has the antiviral, antiinflammatory, cardioprotective and hepatoprotective effect. This study aimed to determine the hepatoprotective activity of Abelmoschus manihot L. Medik leaves extract and its nanoparticles against carbon tetrachloride induced hepatotoxicity in rats.
This was an experimental research. Abelmoschus manihot L. Medik fresh leaves dried and extracted with maceration method using 95% ethanol. The macerate concentrated to obtain a viscous extract. Some extract made into test solutions with a concentration of 8 mg/mL, and some extracts were prepared into nanoparticles with emulsion solvent evaporation method using 0.1% PLGA solution in ethyl acetate and 2.5% PVA solution with a concentration of 0,5 mg/mL. Test animals were 20 male white rats divided into 4 treatment groups ie positive control (Curcuma), negative control (1 % sodium CMC solution), extract group (dose 20 mg/200 g BW) and nanoparticle group (dose 0.25 mg/200 g BW). The rats were treated for 11 days, on the 8th day the rats were induced by CCl4 intraperitoneally with a dose of 1000 mg/1000 g BW. On the 12th day, rats blood were taken and tested for SGOT and SGPT levels and histopathologic observations were performed. Data were analyzed statistically with Anava one way.
The results showed that Abelmoschus manihot L. Medik leaves extract and its nanoparticles lowering the SGOT and SGPT levels. Statistical analysis result showed a significant result (p <0.05) which means that the extract and its nanoparticles have the hepatoprotective activity. The activity of nanoparticles with small dose equals with extract with a larger dose. Rat liver in all treatment groups experienced necrosis, but showed regeration except on negative control group.